MALVERN PANALYTICAL is holding a seminar and practical demonstration of the MICROCAL PEAQ-ITC

Isothermal Titration Calorimetry (ITC) provides the thermodynamic data required for measuring the affinity, stoichiometry and thermodynamics of an interaction. It requires minimal sample preparation and delivers high quality affinity data in a fast, unattended experiment. The high quality of the data and the ease of use of the instrument has resulted in a widespread adoption of the technique in basic protein characterization and early drug discovery programs.

ITC is considered the gold standard for label-free measurement of a wide range of biomolecular interactions for three key reasons: it does not rely on chromophores or fluorophores, it does not require labelling or immobilization of samples, and it has no molecular weight limitations. Instead, ITC depends solely on the direct detection of heat changes upon binding.

This training session, designed for a broad audience,will focus on the benefits of PEAQ-ITC, and solutions to current bottlenecks in interaction analysis.We will also discuss improvements in PEAQ-ITC data quality, enabling greater confidence and resolution when measuring low heats, working with low or uncertain sample concentrations, and analyzing complex binding mechanisms.

In addition, the session will cover practical experimental considerations including how to obtain high-quality data, working with non-aqueous buffers, troubleshooting experiments that do not go as planned, and using the new MicroCal® PEAQ-ITC analysis software. This software enables automated data analysis, reduces user subjectivity, minimizes analysis time, and allows the processing of large datasets (50 or more experiments) in a matter of seconds.